OMIMOnlineMendelianInheritanceinManAll DatabasesPubMedNucleotideProteinGenomeStructurePMCTaxonomyOMIM SearchPubMedProteinNucleotideCoreNucleotideGSSESTStructureGenomeBooksCancerChromosomesConservedDomainsdbGaP3DDomainsGeneGenomeProjectGENSATGEOProfilesGEODataSetsHomoloGeneJournalsMeSHNCBIWebSiteNLMCatalogOMIAOMIMPMCPopSetProbeProteinClustersPubChemBioAssayPubChemCompoundPubChemSubstanceSNPTaxonomyToolKitUniGeneUniSTSLimitsPreview/IndexHistoryClipboardDetailsDisplayTitlesDetailedClinicalSynopsisAllelicVariantsASN1XMLLinkOutRelatedEntriesBookLinksGeneLinksGenomeLinksGEOProfileLinksHomoloGeneLinksNucleotideLinksOMIALinksPubChemBioAssayLinksPMCLinksProteinLinksPubMed(calculated)LinksPubMed(cited)LinksSNPLinksGeneGenotypeLinksStructureLinksUniGeneLinksUniSTSLinksShow5102050100200500SendtoTextFilePrinterClipboardAll1OMIMdbSNP0OMIMUniSTS0*602065LinksADENOSINEDEAMINASERNASPECIFICB2ADARB2AlternativetitlessymbolsADAR3RNAEDITINGENZYME2RATHOMOLOGOFRED2Genemaplocus10p15TEXTDESCRIPTIONRNAeditingdeaminase2(RED2orADARB2)isamemberofthedoublestrandedRNA(dsRNA)adenosinedeaminasefamilyofRNAeditingenzymesAdenosinedeaminationofpremRNAresultsinachangeintheaminoacidsequenceofthegeneproductwhichdiffersfromthatpredictedbythegenomicDNAsequenceOthermembersofthisfamilyincludeDRADA(ADAR601059)andRED1(ADARB1601218)(Mittazetal1997)CLONINGMelcheretal(1996)clonedratRed2Red2is54aminoacidslongerthanRed1attheNterminusBothRed1andDradawereexpressedinbrainandinperipheraltissueshoweverRED2transcriptswerefoundonlyinbrainbyNorthernblotanalysisBysearchingESTdatabasesusingthecatalyticdomainofhumanADAR1(ADAR)Chenetal(2000)identifiedADARB2whichtheycalledADAR3Thededuced739aminoacidproteincontainsanNterminalRdomainfollowedby2dsRNAbindingmotifsandaCterminaldeaminasedomainTheRdomainisanarginineandlysinerichregionthatincludes6consecutivearginineresiduesADAR3shares72%aminoacidsimilaritywithADAR2(ADARB1)andis84%identicaltoratAdar3Northernblotanalysisofseveraltissuesdetectedamajor95kbtranscriptonlyinbrainWithinspecificbrainregionsthalamusandamygdalashowedhighestexpressionWesternblotanalysisoftotalmousebraindetectedAdar3atanapparentmolecularmassof81kDGENEFUNCTIONMelcheretal(1996)foundthatbothratRed1andDradawereabletoeditRNAsencodingglutamatereceptorsubunits(GluRs)buttheyfoundnoeditingactivityofGluRmRNAsforratRed2invitroChenetal(2000)wereunabletodetectRNAeditingactivitybyhumanADAR3consistentwiththeresultsinratHoweverADAR3bounddsRNAandsinglestrandedRNA(ssRNA)DeletionmutagenesisdeterminedthattheNterminalargininerichregionofADAR3mediateditsbindingtossRNAADAR3alsoinhibitedinvitroRNAeditingbyotherADARfamilymembersChenetal(2000)hypothesizedthatADAR3mayhavearegulatoryroleinRNAeditingMAPPINGBysomaticcellhybridanalysisMittazetal(1997)mappedtheRED2genetochromosome10p15REFERENCES1ChenCXChoDSCWangQLaiFCarterKCNishikuraKAthirdmemberoftheRNAspecificadenosinedeaminasegenefamilyADAR3containsbothsingleanddoublestrandedRNAbindingdomainsRNA67557672000PubMedID108367962MelcherTMaasSHerbASprengelRHiguchiMSeeburgPHRED2abrainspecificmemberoftheRNAspecificadenosinedeaminasefamilyJBiolChem27131795317981996PubMedID89432183MittazLAntonarakisSEHiguchiMScottHSLocalizationofanovelhumanRNAeditingdeaminase(hRED2orADARB2)tochromosome10p15HumGenet1003984001997PubMedID9272162CONTRIBUTORSPatriciaAHartzupdated11/5/2004CREATIONDATEClairAFrancomano10/21/1997EDITHISTORYmgross11/10/2004terry11/5/2004alopez11/5/1997dholmes10/22/1997Copyright©19662007JohnsHopkinsUniversityDisplayTitlesDetailedClinicalSynopsisAllelicVariantsASN1XMLLinkOutRelatedEntriesBookLinksGeneLinksGenomeLinksGEOProfileLinksHomoloGeneLinksNucleotideLinksOMIALinksPubChemBioAssayLinksPMCLinksProteinLinksPubMed(calculated)LinksPubMed(cited)LinksSNPLinksGeneGenotypeLinksStructureLinksUniGeneLinksUniSTSLinksShow5102050100200500SendtoTextFilePrinterClipboardDisclaimerWritetotheHelpDeskPrivacyPolicyNCBINLMNIH