OMIMOnlineMendelianInheritanceinManAll DatabasesPubMedNucleotideProteinGenomeStructurePMCTaxonomyOMIM SearchPubMedProteinNucleotideCoreNucleotideGSSESTStructureGenomeBooksCancerChromosomesConservedDomainsdbGaP3DDomainsGeneGenomeProjectGENSATGEOProfilesGEODataSetsHomoloGeneJournalsMeSHNCBIWebSiteNLMCatalogOMIAOMIMPMCPopSetProbeProteinClustersPubChemBioAssayPubChemCompoundPubChemSubstanceSNPTaxonomyToolKitUniGeneUniSTSLimitsPreview/IndexHistoryClipboardDetailsDisplayTitlesDetailedClinicalSynopsisAllelicVariantsASN1XMLLinkOutRelatedEntriesBookLinksGeneLinksGenomeLinksGEOProfileLinksHomoloGeneLinksNucleotideLinksOMIALinksPubChemBioAssayLinksPMCLinksProteinLinksPubMed(calculated)LinksPubMed(cited)LinksSNPLinksGeneGenotypeLinksStructureLinksUniGeneLinksUniSTSLinksShow5102050100200500SendtoTextFilePrinterClipboardAll1OMIMdbSNP0OMIMUniSTS0*601662LinksACTIVATEDLEUKOCYTECELLADHESIONMOLECULEALCAMAlternativetitlessymbolsCD166Genemaplocus3q131q132TEXTCLONINGCD6(186720)isareceptorexpressedonTcellsthatisbelievedtobeinvolvedincelladhesioninteractionsbetweenthymocytesandthymicepithelialcellsBowenetal(1995)observedthatanumberofcelllinesincludinghumanthymicepithelialcellsexpressedaputativeCD6ligandwhichtheypurifiedusingantibodyaffinitypurificationFromthispurifiedCD6ligandtheyobtainedaminoacidsequenceswithsignificanthomologytothechickenneuraladhesionmoleculeBEN/SC1/DMGRASPTheythenusedPCRfragmentsofchickenBENtoscreenhumanactivatedTcellandHL60cellcDNAlibrariestoobtaincDNAclonesThishumanCD6ligandwasnamedALCAMfor'activatedleukocytecelladhesionmolecule'TheALCAMgeneencodesa500aminoacidpolypeptidewithasecretorysignalsequenceand5immunoglobulin(Ig)domainsNorthernblotanalysisdetecteda52kbmRNAexpressedbymitogenactivatedleukocytesandanumberofTcellBcellmonocyteandtumorderivedcelllinesbutnotbyunactivatedleukocytesGENEFUNCTIONBowenetal(1995)demonstratedthatALCAMisaCD6ligandbyshowingthatanALCAMfusionproteinboundtoCOScellstransfectedwithCD6Skonieretal(1996)performedtargetedmutagenesisonALCAMandfoundthattheresiduesconstitutingtheCD6bindingsiteareclusteredononefaceoftheNterminalIgdomainofALCAMTheauthorsobservedthatthesequencesinthisregionareconservedacrossspeciesandthatthisdomainmaybethepreferredsiteforinteractionsofmanycellsurfaceIgsuperfamilymembersandthediversemoleculeswithwhichtheyinteractByimmunoprecipitationanalysisusingaCD6fusionproteinJooetal(2000)detectedbothCD166andanovelantigen3A11onepithelialandmesenchymalcellsUsingFACSanalysisSaifullahetal(2004)showedthatbothCD166and3A11wereexpressedatdifferentlevelsonavarietyofepithelialandmesenchymalcelllinesderivedfromthymusskinsynoviumandcartilageExpressionof3A11butnotCD166wasenhancedbyIFNG(147570)Immunoblotanalysisshowedexpressionofa130kD3A11antigenanda105kDCD166antigenonrheumatoidarthritissynovialfibroblastsConfocalmicroscopyrevealedcoexpressionof3A11andCD166atthecellsurfaceTransfectionofepithelialcellswithCD166specificsmallinterferingRNAsignificantlydecreasedexpressionofCD166withoutaltering3A11antigenlevelsSaifullahetal(2004)concludedthat3A11andCD166aredistinctCD6ligandsTheysuggestedthat3A11maymediateinteractionsofsynovialandepithelialcellswithTlymphocytesMAPPINGByfluorescenceinsituhybridizationtheymappedthehumanALCAMgenetochromosome3q131q132REFERENCES1BowenMAPatelDDLiXModrellBMalackoARWangWCMarquardtHNeubauerMPesandoJMFranckeUHaynesBFAruffoACloningmappingandcharacterizationofactivatedleukocytecelladhesionmolecule(ALCAM)aCD6ligandJExpMed181221322201995PubMedID77600072JooYSSingerNGEndresJLSarkarSKinneRWMarksRMFoxDAEvidencefortheexpressionofasecondCD6ligandbysynovialfibroblastsArthritisRheum433293352000PubMedID106938723SaifullahMKFoxDASarkarSAbidiSMAEndresJPiktelJHaqqiTMSingerNGExpressionandcharacterizationofanovelCD6ligandincellsderivedfromjointandepithelialtissuesJImmun173612561332004PubMedID155283494SkonierJEBowenMAEmswilerJAruffoABajorathJRecognitionofdiverseproteinsbymembersoftheimmunoglobulinsuperfamilydelineationofthereceptorbindingsiteinthehumanCD6ligandALCAMBiochemistry3512287122911996PubMedID8823162CONTRIBUTORSPaulJConverseupdated10/5/2006CREATIONDATEJenniferPMacke2/4/1997EDITHISTORYmgross10/24/2006terry10/5/2006jamie2/4/1997Copyright©19662007JohnsHopkinsUniversityDisplayTitlesDetailedClinicalSynopsisAllelicVariantsASN1XMLLinkOutRelatedEntriesBookLinksGeneLinksGenomeLinksGEOProfileLinksHomoloGeneLinksNucleotideLinksOMIALinksPubChemBioAssayLinksPMCLinksProteinLinksPubMed(calculated)LinksPubMed(cited)LinksSNPLinksGeneGenotypeLinksStructureLinksUniGeneLinksUniSTSLinksShow5102050100200500SendtoTextFilePrinterClipboardDisclaimerWritetotheHelpDeskPrivacyPolicyNCBINLMNIH