OMIMOnlineMendelianInheritanceinManAll DatabasesPubMedNucleotideProteinGenomeStructurePMCTaxonomyOMIM SearchPubMedProteinNucleotideCoreNucleotideGSSESTStructureGenomeBooksCancerChromosomesConservedDomainsdbGaP3DDomainsGeneGenomeProjectGENSATGEOProfilesGEODataSetsHomoloGeneJournalsMeSHNCBIWebSiteNLMCatalogOMIAOMIMPMCPopSetProbeProteinClustersPubChemBioAssayPubChemCompoundPubChemSubstanceSNPTaxonomyToolKitUniGeneUniSTSLimitsPreview/IndexHistoryClipboardDetailsDisplayTitlesDetailedClinicalSynopsisAllelicVariantsASN1XMLLinkOutRelatedEntriesBookLinksGeneLinksGenomeLinksGEOProfileLinksHomoloGeneLinksNucleotideLinksOMIALinksPubChemBioAssayLinksPMCLinksProteinLinksPubMed(calculated)LinksPubMed(cited)LinksSNPLinksGeneGenotypeLinksStructureLinksUniGeneLinksUniSTSLinksShow5102050100200500SendtoTextFilePrinterClipboardAll1OMIMdbSNP0OMIMUniSTS0*609774LinksAPOPTOSISINHIBITOR5API5AlternativetitlessymbolsANTIAPOPTOSISCLONE11AAC11Genemaplocus11p12TEXTCLONINGByscreeningforcDNAsthatpermittedsurvivalofmousefibroblastsinserumfreemediumfollowedbyrescreeningthecDNAlibraryTewarietal(1997)isolatedshortandlongvariantsofApi5whichtheycalledAac11TheyclonedhumanAAC11bydatabaseanalysisandscreeninggingivaandleukemiacDNAlibrariesThededuced504aminoacidhumanproteinsharesahighdegreeofidentitywiththelongmouseproteinAAC11containsseveralproteinphosphorylationsites2NglycosylationsitesandaleucinerichsequencewithweaksimilaritytoaleucinezippermotifNorthernblotanalysisdetectedamajorAac11transcriptinalladultandembryonicmousetissuesexaminedandseveralothertranscriptsexpressedinatissuespecificmannerAAC11expressionwasalsodetectedinseveralhumantumorcelllinesWesternblotanalysisofmousefibroblastsdetectedamajor55kDproteinandaminor25kDproteincorrespondingtothelongandshortAac11isoformsrespectivelyGENEFUNCTIONTewarietal(1997)foundthatmousefibroblastsstablytransfectedwithcDNAencodingtheshortisoformofmouseAac11wereviableinserumfreemediumTheprotectionwasabolishedbymutationofleucinestoarginineswithintheleucinezipperdomainThelongerAac11isoformdidnotprotectagainstapoptosisMAPPINGHartz(2005)mappedtheAPI5genetochromosome11p12basedonanalignmentoftheAPI5sequence(GenBankU83857)withthegenomicsequence(build35)REFERENCES1HartzPAPersonalCommunicationBaltimoreMd12/12/20052TewariMYuMRossBDeanCGiordanoARubinRAAC11anovelcDNAthatinhibitsapoptosisaftergrowthfactorwithdrawalCancerRes57406340691997PubMedID9307294CREATIONDATEPatriciaAHartz12/12/2005EDITHISTORYmgross12/12/2005Copyright©19662007JohnsHopkinsUniversityDisplayTitlesDetailedClinicalSynopsisAllelicVariantsASN1XMLLinkOutRelatedEntriesBookLinksGeneLinksGenomeLinksGEOProfileLinksHomoloGeneLinksNucleotideLinksOMIALinksPubChemBioAssayLinksPMCLinksProteinLinksPubMed(calculated)LinksPubMed(cited)LinksSNPLinksGeneGenotypeLinksStructureLinksUniGeneLinksUniSTSLinksShow5102050100200500SendtoTextFilePrinterClipboardDisclaimerWritetotheHelpDeskPrivacyPolicyNCBINLMNIH