Alternative titles; symbols
ABI2
ABL-INTERACTING PROTEIN 1; AIP1
TEXT
The ABL protein (ABL1; 189980) is the cellular homolog of the Abl oncogene product of Abelson murine leukemia virus. ABL is a tyrosine kinase that may regulate signal transduction events in the cytoplasm and processes in the nucleus. The transforming Abl proteins are constitutively active tyrosine kinases primarily localized in the cytoplasm. They are repressed by SH3 domains and possibly by other regions. 
CLONING
Using a yeast 2-hybrid screen of a lymphocyte cDNA library with the ABL1 SH3 domain as bait, Dai and Pendergast (1995) isolated a cDNA encoding ABI2. Sequence analysis predicted that the 401-amino acid ABI2 protein is 65% identical to the Abi1 mouse protein and 70% identical to the frog Xlan4 protein. ABI2 possesses a basic N terminus with homology to a homeodomain protein; a central serine-rich region; 3 PEST sequences, which are implicated in susceptibility to protein degradation; several proline-rich stretches; and an acidic C terminus with multiple phosphorylation sites and an SH3 domain. Northern blot analysis revealed ubiquitous but variable expression of 7.0- and 1.9-kb ABI2 transcripts. Binding and mutational analyses showed that the interaction between the N-terminal proline-rich stretch of ABI2 and the ABL1 SH3 domain is highly specific, but that the ABI2 SH3 domain also binds to a proline-rich stretch downstream of the nuclear localization signal in the ABL1 C terminus. Immunoblot and autoradiographic analyses indicated that ABI2 is phosphorylated by ABL1. ABI2 mutants lacking the N-terminal proline-rich ABL1 SH3 domain-binding residues activated the transforming capacity of ABL1. Dai and Pendergast (1995) suggested that the ABI proteins may function to coordinate the cytoplasmic and nuclear functions of the ABL1 tyrosine kinase.
MAPPING
By analysis of a YAC and a BAC, Machado et al. (2000) mapped the ABI2 gene to 2q31-q33.
REFERENCES
- 1. Dai, Z.; Pendergast, A. M. :
- Abi-2, a novel SH3-containing protein interacts with the c-Abl tyrosine kinase and modulates c-Abl transforming activity. Genes Dev. 9: 2569-2582, 1995.
PubMed ID : 7590236
- 2. Machado, R. D.; Pauciulo, M. W.; Fretwell, N.; Veal, C.; Thomson, J. R.; Guell, C. V.; Aldred, M.; Brannon, C. A.; the International PPH Consortium; Trembath, R. C.; Nichols, W. C. :
- A physical and transcript map based upon refinement of the critical interval for PPH1, a gene for familial primary pulmonary hypertension. Genomics 68: 220-228, 2000.
PubMed ID : 10964520
CREATION DATE
Paul J. Converse : 11/7/2001
EDIT HISTORY
mgross : 11/7/2001
mgross : 11/7/2001
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