Alternative titles; symbols
SHREW1
Gene map locus
1p36.32TEXT
CLONING
Using differential display RT-PCR to identify cDNAs specific to invasive versus noninvasive derivatives of an endometriotic cell line, followed by database analysis and 5-prime and 3-prime RACE of a brain cDNA library, Bharti et al. (2004) cloned AJAP1, which they called SHREW1. The predicted 411-amino acid protein is highly alkaline and has a calculated molecular mass of 43 kD. It contains an N-terminal signal peptide, a transmembrane domain, and potential sites for phosphorylation, glycosylation, and myristoylation. Database analysis suggested that SHREW1 may be restricted to vertebrates. Northern blot analysis of invasive endometriotic cells detected a 4-kb SHREW1 transcript. Immunoblot analysis of human pancreas and uterus revealed an endogenous 48-kD SHREW1 protein. Epitope-tagged SHREW1 localized to the basolateral membrane of polarized epithelial cells and colocalized with E-cadherin (CDH1; 192090), a marker of adherens junctions. 
GENE FUNCTION
Schreiner et al. (2007) found that overexpression of SHREW1 protein enhanced invasiveness of human fibrosarcoma cells. SHREW1 interacted with CD147 (BSG; 109480), a mediator of invasiveness and matrix metalloprotease activity, in epithelial cells. Downregulation of SHREW1 or CD147 using small interfering RNA in HeLa cells decreased invasiveness without affecting proliferation. Schreiner et al. (2007) concluded that SHREW1-CD147 interaction is associated with regulation of cellular invasion.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the AJAP1 gene to chromosome 1 (SGC32361).
REFERENCES
- 1. Bharti, S.; Handrow-Metzmacher, H.; Zickenheiner, S.; Zeitvogel, A.; Baumann, R.; Starzinski-Powitz, A. :
- Novel membrane protein shrew-1 targets to cadherin-mediated junctions in polarized epithelial cells. Molec. Biol. Cell 15: 397-406, 2004.
PubMed ID : 14595118
- 2. Schreiner, A.; Ruonala, M.; Jakob, V.; Suthaus, J.; Boles, E.; Wouters, F.; Starzinsi-Powitz, A. :
- Junction protein shrew-1 influences cell invasion and interacts with invasion-promoting protein CD147. Molec. Biol. Cell 18: 1272-1281, 2007.
PubMed ID : 17267690
CREATION DATE
Alan F. Scott : 4/24/2007
EDIT HISTORY
mgross : 4/24/2007
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