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ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 21; ANKRD21

Alternative titles; symbols

PROSTATE-, OVARY-, TESTIS-, AND PLACENTA-EXPRESSED GENE; POTE
PROSTATE-, OVARY-, TESTIS-, AND PLACENTA-EXPRESSED GENE ON CHROMOSOME 21; POTE21

Gene map locus 21q11.2

TEXT

CLONING

By screening an EST database for prostate-specific genes, Bera et al. (2002) identified POTE. They cloned the full-length POTE cDNA from a prostate cDNA library. The deduced 584-amino acid protein has a calculated molecular mass of 66.4 kD. POTE contains 5 to 7 ankyrin repeats; a putative transmembrane region, which is in between, and partly overlaps with, ankyrin repeats 5 and 6; and an alpha-helical C terminus. RT-PCR and multitissue dot blot analysis detected robust expression in prostate and testis and weak expression in placenta and ovary. Northern blot analysis detected a 2.0-kb transcript in prostate and testis. POTE was also expressed in a leukemia cell line and in an androgen-responsive prostate cancer cell line; it was not detected in an androgen-unresponsive prostate cancer cell line. In situ hybridization detected a POTE transcript expressed in the basal and terminal epithelial cells of normal prostate epithelium and in prostate cancer cells. 30 PubMed Neighbors

GENE STRUCTURE

Bera et al. (2002) determined that the POTE gene contains 11 exons and spans about 32 kb.

MAPPING

By genomic sequence analysis and FISH, Bera et al. (2002) mapped the POTE gene to chromosome 21q11.2. FISH detected paralogs of POTE on chromosomes 2 (608914), 8 (608915), 13, 14 (608916), 15 (608912), 18, and 22 (608913).

REFERENCES

1. Bera, T. K.; Zimonjic, D. B.; Popescu, N. C.; Sathyanarayana, B. K.; Kumar, V.; Lee, B.; Pastan, I. :
POTE, a highly homologous gene family located on numerous chromosomes and expressed in prostate, ovary, testis, placenta, and prostate cancer. Proc. Nat. Acad. Sci. 99: 16975-16980, 2002. Note: Erratum: Proc. Nat. Acad. Sci. 100: 1462 only, 2003.
PubMed ID : 12475935

CREATION DATE

Patricia A. Hartz : 2/6/2003

EDIT HISTORY

mgross : 9/22/2005
mgross : 9/13/2004
alopez : 5/13/2004
mgross : 2/25/2003
mgross : 2/6/2003

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